Predicted vs. Validated Stabilizing Positions

BsLipA (PDB 1ISP) — 20 experimentally confirmed positions, colored by prediction outcome after calibration

Protein surface Cartoon backbone Labels Spheres

Found, correct amino acid (11)

Found, different amino acid (7)

Not found (2)

Catalytic triad

18/20 positions recovered (90%) · 11 exact amino acid matches · All 4 highest-impact mutations found